Spectrophotometric measurement of hydroperoxides at increased sensitivity by oxidation of Fe2+ in the presence of xylenol orange
β Scribed by Deiana, Luca; Carru, Ciriaco; Pes, Gianni; Tadolini, Bruna
- Book ID
- 115447030
- Publisher
- Informa plc
- Year
- 1999
- Tongue
- English
- Weight
- 634 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1071-5762
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We describe the application of the FOX2 (ferrous oxidation in xylenol orange, version 2) method to the measurement of hydroperoxides in plasma. Authentic plasma hydroperoxides can be determined by a strategy in which the hydroperoxide reductant, triphenylphosphine, is used to discriminate between th
A simple and sensitive method for the direct measurement of lipid peroxides in lipoprotein and liposomes is described. The method is based on the principle of the rapid peroxide-mediated oxidation of Fe2+ to Fe3+ under acidic conditions. The latter, in the presence of xylenol orange, forms a Fe(3+)-