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Spectrophotometric evidence for a glutamate dehydrogenase-l-leucine complex

โœ Scribed by Russell A. Prough; Johanna M. Culver; Harvey F. Fisher


Book ID
115701461
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
389 KB
Volume
149
Category
Article
ISSN
0003-9861

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Specific messenger RNA for glutamate dehydrogenase was partially purified from a calf liver polysomal poly(A)-mRNA fraction by sucrose density gradient centrifugation. Enzyme activity in the translational incubation mixture was detected by measuring NADH oxidation in the presence of c~-ketoglutarate