✦ LIBER ✦

Identification of messenger RNA for glutamate dehydrogenase using a spectrophotometric probe

✍ Scribed by M. Hillar; S. Orudiakomo

Book ID: 104768192
Publisher: Springer
Year: 1986
Tongue: English
Weight: 327 KB
Volume: 11
Category: Article
ISSN: 0301-4851
DOI: 10.1007/bf00419732

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✦ Synopsis

Specific messenger RNA for glutamate dehydrogenase was partially purified from a calf liver polysomal poly(A)-mRNA fraction by sucrose density gradient centrifugation. Enzyme activity in the translational incubation mixture was detected by measuring NADH oxidation in the presence of c~-ketoglutarate and ammonia as a decrease in absorbancy 340-442 nm in a dual wavelength Aminco DW-2 spectrophotometer.

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