Spectrophotometric determination of pyridoxine hydrochloride (vitamin B6) in multivitamin preparations

✍ Scribed by R. C. Shah; P. V. Raman; M. M. Mehta

• Publisher: John Wiley and Sons
• Year: 1965
• Tongue: English
• Weight: 463 KB
• Volume: 54
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600540319

✦ Synopsis

A spectrophotometric procedure has been developed for rapid and fairly accurate determination of vitamin BE in B complex and multivitamin preparations. Vitamin BE is determined by the measurement of difference in absorbance value (AA) in different solvents at 328 mp.

HE PRESENT official U.S.P. method (I) for the Tdetermination of vitamin B6 in multivitamin preparations is the colorimetric method, consisting of the coupling of vitamin Ba with 2,6-dichloroquinone-chlorimide in ammonium chlorideammonium hydroxide buffer. There are also many other methods for the colorimetric determination of vitamin Be. Pyridoxine reacts with Folin-Denis reagent to produce a blue color (2). It couples with diazo compounds, such as diazotized sulfanilic acid (3) and diazotized p-aminoacetophenone (4, 5). The method of Sweeny and Hall (6) can be used to determine pyridoxine, pyridoxal, and pyridoxamine b y suitable modifications.