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Spectrophotometric determination of lysine in the presence of other amino acids and proteins using furfuraldehyde

✍ Scribed by Ibolya Molnár-Perl; Margit Pintér-Szakás

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
577 KB
Volume
257
Category
Article
ISSN
0003-2670

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✦ Synopsis

The selective reaction of lysme, S-bydro&sme, omlthme and cu,e-dlammopunehc acid ~th furfuraldehyde, m anhydrous me&a, was mvestlgated at SO, 85,90 and 95°C by spectrophotometry, 111 the presence of various addItIonal materials,, such as potassmm pyrosulphlte, ascorbic acid and lead(I1) acetate Second-order rate constants and molar absorptiwtles were determmed Optimum condlhons were estabhshed for the selective detemunatlon of lysme m the presence of mtact protems or m protem hydrolysates m the presence of a wde range of other ammo acids mcludmg omlthme Under optimum condltlons, m the concentration range 2 X 10m8-3 X lo-' M, molar absorptlvltles and reproduclhhty data were measured mth a standard error of 0 9-5 0%, dependmg on the procedure applied and on the amount of lysme present &ywor& Spectrophotometry, Ammo acids, Lysme, Proteins

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