Spectrophotometric determination of hydrogen peroxide by using the cleavage of Eriochrome black T in the presence of peroxidase
β Scribed by Min Zhu; Xuemei Huang; Liuzhan Liu; Hanxi Shen
- Book ID
- 117224095
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 257 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0039-9140
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## Abstract In this work a new spectrofluorimetric method for the determination of vitamin B~1~, based on the catalytic activity of horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H~2~O~2~), has been developed. Nonβfluorescent vitamin B~1~ was easily converted through catalytic o
The catalytic activity of carbon blacks for the decomposition of aqueous hydrogen peroxide under given conditions of time, temperature and concentration depends on surface alkalinity and surface area of the blacks. A small fraction only of the total oxygen evolved in the process gets chemisorbed by