Spectrofluorimetric determination of vitamin B1 using horseradish peroxidase as catalyst in the presence of hydrogen peroxide

✍ Scribed by Mir Azam Khan; Seung Oh Jin; Sang Hak Lee; Hye Young Chung

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 233 KB
• Volume: 24
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1067

✦ Synopsis

Abstract

In this work a new spectrofluorimetric method for the determination of vitamin B~1~, based on the catalytic activity of horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H~2~O~2~), has been developed. Non‐fluorescent vitamin B~1~ was easily converted through catalytic oxidation in alkaline medium into a fluorescent compound, even without exposure to light. The linear range for vitamin B~1~ observed was 0.026–16.83 µg/mL (RSD = 1.75%). The correlation coefficient for the calibration curve and limit of detection were found to be 0.9964 and 0.015 µg/mL, respectively. The developed method is practical, simple, sensitive and relatively free from interference by coexisting substances and has been successfully applied for the determination of vitamin B~1~ in pharmaceutical preparations. Copyright © 2008 John Wiley & Sons, Ltd.