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Spectrophotometric determination of enzymatically generated hydrogen peroxide using Sol-Gel immobilized horseradish peroxidase

✍ Scribed by Faida A. El-Essi; Ali Z.Abu Zuhri; Suleiman I. Al-Khalil; Monzir S. Abdel-Latif

Book ID: 117224115
Publisher: Elsevier Science
Year: 1997
Tongue: English
Weight: 589 KB
Volume: 44
Category: Article
ISSN: 0039-9140
DOI: 10.1016/s0039-9140(97)00032-5

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## Abstract In this work a new spectrofluorimetric method for the determination of vitamin B~1~, based on the catalytic activity of horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H~2~O~2~), has been developed. Non‐fluorescent vitamin B~1~ was easily converted through catalytic o