Spectrophotometric determination of amikacin, kanamycin, neomycin and tobramycin
โ Scribed by Confino, Maya ;Bontchev, Panayot
- Publisher
- Springer-Verlag
- Year
- 1990
- Weight
- 240 KB
- Volume
- 102
- Category
- Article
- ISSN
- 0344-838X
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The reaction of 2.4-dInitrofluorobenzcne (Sanger's rcagcnt) is used to form colored products with aminoglycoside antibiotics. Stopping the progress of the reaction with acid after a fixed time allows aqueous solubility to be maintained while discharging any color due to excess reactant. Thechoice of
## Abstract A simple and selective micellar electrokinetic chromatography (MEKC) with UV detection is described for simultaneous determination of amikacin, tobramycin, and kanamycin A, performed in Tris buffer (180ย mM; pH 9.1) with 300ย mM sodium pentanesulfonate (SPS) as an anionic surfactant. Unde