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Colorimetric determination of gentamicin, kanamycin, tobramycin, and amikacin aminoglycosides with 2,4-dinitrofluorobenzene

✍ Scribed by James A. Ryan

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 187 KB
Volume: 73
Category: Article
ISSN: 0022-3549
DOI: 10.1002/jps.2600730930

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✦ Synopsis

The reaction of 2.4-dInitrofluorobenzcne (Sanger's rcagcnt) is used to form colored products with aminoglycoside antibiotics. Stopping the progress of the reaction with acid after a fixed time allows aqueous solubility to be maintained while discharging any color due to excess reactant. Thechoice of an appropriate analytical wavelength results in adherence to Beer's law. Although this colorimetric method is not expected to be stability-indicating, it is convenient and should be useful in content uniformity dcterminations for pharmaceutical dosage forms (e.g., ointments).

Keyphrases 0 Colorimetry-aminoglycosides, 2.4-dinitrofluorobenzene 0 Aminoglycosides-2,4-dinitrofluorobenzene, colorimetric determination

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