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Spectrophotometric assay and electrophoretic detection of trans-feruloyl esterase activity

✍ Scribed by John A. McCallum; Iain E.P. Taylor; G.H.Neil Towers


Book ID
107718978
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
967 KB
Volume
196
Category
Article
ISSN
0003-2697

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A direct spectrophotometric pH indicator method has been devised to assay the activity of the D-Ala carboxypeptidase/transpeptidase of Streptomyces R61, and which should be of general application to D-Ala carboxypeptidases. The substrate employed is N,N'diacetyl-r.lysyl-D-alanyl-D-lactate. The metho