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A direct spectrophotometric assay for d-alanine carboxypeptidases and for the esterase activity of β-lactamases

✍ Scribed by R.F. Pratt; W.Stephen Faraci; Chandrika P. Govardhan

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
258 KB
Volume
144
Category
Article
ISSN
0003-2697

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✦ Synopsis

A direct spectrophotometric pH indicator method has been devised to assay the activity of the D-Ala carboxypeptidase/transpeptidase of Streptomyces R61, and which should be of general application to D-Ala carboxypeptidases. The substrate employed is N,N'diacetyl-r.lysyl-D-alanyl-D-lactate. The method allows the determination of steady-state kinetic parameters, and can also be used for the assay of the esterase activity of Blactamases against specific depsipeptides. 0 1985 Academic prss. Inc.

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