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Spectrofluorimetric flow-injection determination of calcium using calcein

✍ Scribed by N. Chimpalee; D. Chimpalee; R. Jarungpattananon; S. Lawratchavee; D.Thorburn Burns

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 305 KB
Volume: 271
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(93)80052-m

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✦ Synopsis

Calcium can be determined spectrofluornnetrlcally (Aem = 520 nm, A, = 365 nm) based on Its reaction wth Calcem m strongly alkahne solution m a flow-m]ectlon system The tamer stream was delomsed water and the reagent streams were 06 M potassmm hydroxide and 60 kg ml -* Calcem The mJectlon rate was 50 h-' The cahbratlon graph was slgmold, but, however, linear between 3 0 and 7 0 kg ml -' Ca based on mJectlon volumes of 250 ~1 The relative standard deviation (n = 10) was 0 23% for 4 pg ml -* Ca The system has been applied to the determmatlon of calcmm m certified reference mineral samples

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