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Spectrofluorimetric Determination of Trace Amounts of Tyrosine with 1,5-Bis(4,6-dicholro-1,3,5-triazinylamino) naphthalene

✍ Scribed by Wei Wei; Wang Hong-Jian; Jiang Chong-Qiu; Shi Jing-Min

Publisher: Elsevier Science
Year: 2007
Tongue: Chinese
Weight: 200 KB
Volume: 35
Category: Article
ISSN: 1872-2040
DOI: 10.1016/s1872-2040(08)60008-0

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✦ Synopsis

1,5-Bis(4,6-dicholro-1,3,5-triazinylamino)

naphthalene was synthesized as a completely new fluorescent reagent and with it a novel fluorescent method was developed for the determination of tyrosine. Under pH 12.0, the fluorescent intensity at λ ex /λ em = 400 nm/465 nm was directly proportional to the concentration of tyrosine in the range of 1.1 × 10 -7 -1.1 × 10 -5 M with the detection limit of 6.8×10 -8 M. The proposed method has been successfully applied to the determination of trace amounts of tyrosine in human serum and urine samples.

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