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Spectral Differentiation and Immunoaffinity Capillary Electrophoresis Separation of Enantiomeric Benzo( a )pyrene Diol Epoxide-Derived DNA Adducts

✍ Scribed by Miksa, Beata; Chinnappan, Raja; Dang, Nhan C.; Reppert, Mike; Matter, Brock; Tretyakova, Natalia; Grubor, Nenad M.; Jankowiak, Ryszard

Book ID: 126046978
Publisher: American Chemical Society
Year: 2007
Tongue: English
Weight: 146 KB
Volume: 20
Category: Article
ISSN: 0893-228X
DOI: 10.1021/tx7001096

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## Abstract Benzo[__a__]pyrene diol epoxide (BPDE) was reacted __in vitro__ with (2'‐deoxy)nucleotides and with calf thymus DNA. The modified DNA was enzymatically hydrolyzed to the 5'‐monophosphate nucleotides using deoxyribonuclease I (DNA‐ase I), nuclease P1 and snake venom phosphodiesterase (SV