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Detection and Identification of Benzo[ a ]pyrene Diol Epoxide Adducts to DNA Utilizing Capillary Electrophoresis-Electrospray Mass Spectrometry

✍ Scribed by Barry, John P.; Norwood, Curt; Vouros, Paul


Book ID
127373645
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
233 KB
Volume
68
Category
Article
ISSN
0003-2700

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Analysis of benzo[a]pyrene diol epoxide-
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## Abstract Benzo[__a__]pyrene diol epoxide (BPDE) was reacted __in vitro__ with (2'‐deoxy)nucleotides and with calf thymus DNA. The modified DNA was enzymatically hydrolyzed to the 5'‐monophosphate nucleotides using deoxyribonuclease I (DNA‐ase I), nuclease P1 and snake venom phosphodiesterase (SV