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Specificity of lens initiator tRNA for N-terminal recognition

✍ Scribed by G. J. A. M. Strous; H. Bloemendal

Publisher
Springer
Year
1973
Tongue
English
Weight
320 KB
Volume
1
Category
Article
ISSN
0301-4851

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✦ Synopsis

The optimal magnesium ion concentration for chain initiation in a cell-free system derived from bovine eye lens which synthesizes 4 classes of crystallins appears to be 5 mM. In the synthesis of α-crystallin polypeptides which contain one internal methionine residue and the second one in N-terminal position, Met-RNA(fMet) functions exclusively as initiator. On the other hand at 5 mM Mg(2+) Met-tRNA(Met) inserts its methionine into the internal position. However, at higher magnesium ion concentrations the initiator tRNA also donates methionine for chain elongation while at the same time the cell-free system loses its capacity to initiate new polypeptides.

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