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Specificity of an esterase (XYLD) from Pseudomonas fluorescens subsp. cellulosa

✍ Scribed by Craig B. Faulds; Marie-Christine Ralet; Gary Williamson; Geoffrey P. Hazlewood; Harry J. Gilbert


Book ID
116126768
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
331 KB
Volume
1243
Category
Article
ISSN
0304-4165

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Directed Evolution of an Esterase from P
✍ Henke, E.; Bornscheuer, U. T. πŸ“‚ Article πŸ“… 1999 πŸ› Walter de Gruyter GmbH & Co. KG 🌐 English βš– 165 KB

The gene encoding an esterase from Pseudomonas fluorescens (PFE) was subjected to random mutagenesis by error-prone PCR or by using the mutator strain Epicurian coli XL1-Red. Enzyme libraries were then created in microtiter plates by expression of PFE-variants in E. coli. These were assayed for impr