Directed Evolution of an Esterase from P
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Henke, E.; Bornscheuer, U. T.
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Article
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1999
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Walter de Gruyter GmbH & Co. KG
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English
โ 165 KB
The gene encoding an esterase from Pseudomonas fluorescens (PFE) was subjected to random mutagenesis by error-prone PCR or by using the mutator strain Epicurian coli XL1-Red. Enzyme libraries were then created in microtiter plates by expression of PFE-variants in E. coli. These were assayed for impr