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Specific Y chromoome fluorescence in interphase nuclei of epithelial cells in human buccal smears

โœ Scribed by A. H. Siddiqui; A. M. P. Janssen de Limpens; R. H. Cormane

Publisher
Springer-Verlag
Year
1978
Tongue
English
Weight
514 KB
Volume
262
Category
Article
ISSN
0340-3696

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โœฆ Synopsis

A simplified method is described to demonstrate Y chromosome fluorescence in interphase nuclei of epithelial cells in human buccal smears using Quinacrine Mustard. This technique turned out to be efficient in our hands and would be of value in determining the sex chromosomes in the epithelial cells of the skin in cases of allogeneic transplantations from females to males and vice versa to determine the peroid during which the grafts survived.

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Application of fluorescent in situ hybridization with X and Y chromosome specific probes to buccal smear analysis
โœ Schad, Chris R.; Kuffel, Daniel G.; Wyatt, William A.; Zinsmeister, Alan R.; Jen ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 572 KB

Conventional Xand Y-chromatin and fluorescent in situ hybridization (FISH) analysis based on Xand Y-chromosome specific probes were conducted from buccal smear, on 15 normal males, 15 normal females, and 9 cases suspected of sex chromosome anomalies. The proportion of Xand Y-chromatin in normal fema