Real-Time, Sequence-Specific Detection o
โ
James G. Nadeau; J.Bruce Pitner; C.Preston Linn; James L. Schram; Cheryl H. Dean
๐
Article
๐
1999
๐
Elsevier Science
๐
English
โ 140 KB
Strand displacement amplification (SDA) is an isothermal nucleic acid amplification method based on the primer-directed nicking activity of a restriction enzyme and the strand displacement activity of an exonuclease-deficient polymerase. Here we describe fluorogenic reporter probes that permit realt