Specific screening methods for glucosinolates in sprout extracts using on-line thermospray liquid chromatography-tandem mass spectrometry

✍ Scribed by C.E.M. Heeremans; R.A.M. Van Der Hoeven; W.M.A. Niessen; J. Vuik; R.H. De Vos; J. Van Der Greef

• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 697 KB
• Volume: 472
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94108-6

✦ Synopsis

Considering the growing importance of the determination and identification of (desulpho-)glucosinolates in extracts of plant materials, rapid screening methods for desulphoglucosinolates were developed, based on thermospray liquid chromatography-mass spectrometry (LC-MS) and LCMS-MS.

In thermospray LC-MS the screening of plant extracts containing various desulphoglucosinolates can be based on the group-specific fragment at m/z 214, but identification of the peaks is not always straightforward owing to the lack of compound-specific fragments in the mass spectra. Using a constant neutral loss of 162 a.m.u., corresponding to the loss of the sugar ring, in thermospray LC-MS-MS the screening and identification of the various desulphoglucosinolates gives better results, especially because the signal in the MS-MS mode is less dependent on the fluctuating thermospray conditions. In some applications direct thermospray MS-MS analysis of mixtures, injected in the flow-injection mode, can give satisfactory results.