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Specific RT-PCR procedure for the detection of human parechovirus type 1 genome in clinical samples

✍ Scribed by Vincent Legay; Jean Jacques Chomel; Bruno Lina


Book ID
114139806
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
121 KB
Volume
102
Category
Article
ISSN
0166-0934

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## Abstract A two‐step reverse transcriptase TaqManβ„’ duplex PCR (RT‐PCR) assay was developed using the ABI 7700 Sequence Detection System for the detection of enterovirus (EV) and parechovirus type 1 and 2 (PEV) RNA from samples of cerebrospinal fluid (CSF) and throat swabs. Using sequence‐specific