A rapid RT-PCR assay for the detection of HIV-1 in human plasma specimens
β Scribed by Burchard, Paul R.; Abou Tayoun, Ahmad N.; Scherer, Axel; Tsongalis, Gregory J.
- Book ID
- 125799965
- Publisher
- Elsevier Science
- Year
- 2014
- Tongue
- English
- Weight
- 617 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0014-4800
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A quantitative human immunodeficiency virus type 1 (HIV-1) RNA polymerase chain reaction assay has been validated analytically and clinically in > 13,000 samples. The assay is highly reproducible with intra-and inter-assay precision of 16% and 19%, respectively. In 1,542 of 1,548 subjects with CD4'
## Abstract A simple and rapid assay for the quantification of infectious HIVβ1 in plasma was developed using shortβterm culture and DNA PCR. This method, called __culture PCR__, allows detection and quantification of infectious HIVβ1 viraemia within 48 hours, and measures the number of infectious