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A rapid RT-PCR assay for the detection of HIV-1 in human plasma specimens

✍ Scribed by Burchard, Paul R.; Abou Tayoun, Ahmad N.; Scherer, Axel; Tsongalis, Gregory J.


Book ID
125799965
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
617 KB
Volume
97
Category
Article
ISSN
0014-4800

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## Abstract A simple and rapid assay for the quantification of infectious HIV‐1 in plasma was developed using short‐term culture and DNA PCR. This method, called __culture PCR__, allows detection and quantification of infectious HIV‐1 viraemia within 48 hours, and measures the number of infectious