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Specific labelling of replicating SPP1 DNA

✍ Scribed by K. J. Burger; T. A. Trautner

Publisher
Springer
Year
1978
Tongue
English
Weight
635 KB
Volume
166
Category
Article
ISSN
1617-4615

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✦ Synopsis

Specific labelling of replicating bacteriophage SPP1 DNA can be achieved by infection at nonpermissive temperature of a B. subtilis strain carrying the initation mutation dnaB ts134. Under these conditions host DNA synthesis is reduced by 90 to 95%. This technique was used to identify cistrons of SPP1 involved in phage DNA synthesis and to define intermediates in SPP1 replication.

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