𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Membrane binding of bacteriophage SPP1 DNA

✍ Scribed by Burger, K. J.


Publisher
Springer
Year
1980
Tongue
English
Weight
330 KB
Volume
179
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis


A fast sedimenting complex was isolated from B. subtilis cells infected with bacteriophage SPP1 by renografin centrifugation. This complex was identified as membrane bound parental and replicating SPP1 DNA. Synthesis of SPP1 DNA takes place in close association with the membrane. This newly synthesized DNA is then released into the cytoplasm. During release, concatemeric SPP1 DNA is sized into monomeric DNA molecules.


πŸ“œ SIMILAR VOLUMES


Gene expression of bacteriophage SPP1
✍ Esche, H. ;Schweiger, M. ;Trautner, T. A. πŸ“‚ Article πŸ“… 1975 πŸ› Springer 🌐 English βš– 683 KB
Gene expression of bacteriophage SPP1
✍ Esche, Helmut πŸ“‚ Article πŸ“… 1975 πŸ› Springer 🌐 English βš– 751 KB

The expression of late SPP1 genes depends on preceding SPP1 DNA replication. This is shown in nonpermissive infection with a mutant defective in DNA replication and after inhibition of DNA synthesis by HPUra. The potential for host gene expression is not significantly influenced by SPP1 infection, a

Specific labelling of replicating SPP1 D
✍ K. J. Burger; T. A. Trautner πŸ“‚ Article πŸ“… 1978 πŸ› Springer 🌐 English βš– 635 KB

Specific labelling of replicating bacteriophage SPP1 DNA can be achieved by infection at nonpermissive temperature of a B. subtilis strain carrying the initation mutation dnaB ts134. Under these conditions host DNA synthesis is reduced by 90 to 95%. This technique was used to identify cistrons of SP

Binding of proflavin to T2L bacteriophag
✍ Philip J. McCall; Victor A. Bloomfield πŸ“‚ Article πŸ“… 1976 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 684 KB

## Abstract We have measured the binding equilibria of proflavin to T2L bacteriophage, in both β€œslow” and β€œfast” sedimenting forms, and to free T2L DNA. Measurements were carried out by difference spectroscopy at 430 nm at temperatures from 13 to 43Β°C and at pH 5.6 and 7.6. We found no significant

SPP1 DNA replicative forms: Growth of ph
✍ Mastromei, Giorgio ;Riva, Silvano ;Fietta, Anna ;Pagani, Laura πŸ“‚ Article πŸ“… 1978 πŸ› Springer 🌐 English βš– 726 KB

The development of bacteriophages SPP1 and phi 29 has been studied in several B. sutilis mutants defective in host DNA replication, under non permissive conditions. Several gene products, involved in the synthesis of host DNA, are required for phi 29 replication, while SPP1 seems to require only the