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Specific Labeling of Isoprenylated Proteins: Application to Study Inhibitors of the Post-translational Farnesylation and Geranylgeranylation

✍ Scribed by R. Danesi; C.A. Mclellan; C.E. Myers


Book ID
115576024
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
422 KB
Volume
206
Category
Article
ISSN
0006-291X

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Synthesis of high specific activity trit
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A small-scale synthesis of high specific activity, N-(2-chloro-2-[3H]-ethyl)-N'-cyclohexyl-N-nitrosourea ([3H]-CCNU) has been accomplished from tritium-labelled ethanolamine. The product is pure by TLC and HPLC analysis and has been used successfully to modify DNA. The overall yield on radioactivity