Specific chromosome changes associated with viral transformation of rat glial cells

✍ Scribed by Kyoko Kano-Tanaka; Tatsuya Tanaka

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: French
• Weight: 675 KB
• Volume: 30
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910300417

✦ Synopsis

Abstract

Karyotypes of three malignant cell lines derived from Wistar and WKA/Mk fetal rat glioblasts, transformed by murine sarcoma virus (MSV‐M‐os) as well as those of four cell lines derived from C6 glioma cells of Wistar origin, retransformed by MSV‐M‐os, were analyzed in early culture passages. The C6 line had a modal number of 42 chromosomes with a normal male karyotype, and only a minor population of cells with 43 chromosomes. The modal chromosome number in every transformed glial cell line shifted from 42 to 43. The G‐banding pattern revealed consistent chromosome abnormalities. Structural chromosome changes occurred in one chromosome No. 2 (2q‐) and in one No. 4 (4q+). The cells with a 43 chromosome karyotype showed trisomy of chromosome No. 12 and its heteromorphism, a finding also confirmed by silver staining. Identical chromosome changes were found in transformed C6 cell lines. A further interesting feature was that all malignant cells had different distribution patterns of silver‐stained nucleolar organizer regions (Ag‐NORs) among particular chromosomes (Nos. 3, 11 and 12) from normal cells, showing an increased frequency of chromosome No. 12 with Ag‐NORs. These results suggested that the gain and/or loss of specific segments involved in chromosomes Nos. 2, 4 and 12 contain(s) genes favorable to malignant transformation in rat glial cells.