Trisomy of chromosome 9q: Specific chromosome change associated with tumorigenicity during the process of X-ray-induced neoplastic transformation in golden hamster embryo cells
✍ Scribed by Keiji Suzuki; Naomi Yasuda; Fumio Suzuki; Osamu Nikaido; Masami Watanabe
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- French
- Weight
- 770 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
We have reported that trisomy of chromosome 7 is commonly observed in anchorage-independent clones isolated from X-irradiated golden hamster embryo cells. All 10 clones derived from different irradiated populations showed tumorigenicity when I X lo7 cells were injected s . ~. into nude mice (BALBk, nuhu). From karyotypic analysis, we found that 8 of 10 cells showed trisomy of chromosome 9. One cell line had a translocation between chromosomes 9q and 19q and trisomy of chromosome 7. The other cell line contained trisomy of chromosome 9 and a translocation between chromosomes 7q and 8q. Using Southern blot analysis, we observed no amplification of v-myc, v-Ha-ras, v-Ki-ras or N-ras-related oncogenes. Furthermore, we could not detect either an increase in expression of v-myc-and v-Ha-ras-related genes or the activation of any oncogene, by the NIH 3T3 transfection assay.
Our results suggest that trisomy of chromosome 7 is insufficient for the expression of tumorigenicity and that increased dosage of chromosome 9q may play an important role in the malignant progression of X-ray-induced neoplastic transformation.