A non-tumorigenic epithelial cell line designated PCIAA, derived from a large pre-malignant colorectal adenoma from a patient with familial polyposis coli (also referred to as hereditary adenomatosis of the colon and rectum) has become immortal in vitro. PCIAA has been passaged in vitro continuously
Specific chromosome abnormalities in human colorectal adenoma epithelial cell lines: Possible role of chromosome 1 in in vitro immortalization
β Scribed by C. Paraskeva; S. Finerty; R.A. Mountford; A. Harvey; S.C. Powell
- Book ID
- 119104370
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 68 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0165-4608
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We have previously reported that continuous in vitro passage in the presence of 3T3 feeders of a non-tumorigenic adenoma-derived epithelial cell line, designated PCIAA, resulted in its becoming immortal. At early passage PCIAA was normal diploid, whereas every cell of PCIAA late passage had an isoch
Bone-marrow chromosomes were examined from 38 mentally and physically retarded and two psychiatric patients who were being treated with a variety of neuropharmacologic drugs. Twenty of these patients used clozapine (Leponex). The clastogenic effects of clozapine in vitro were studied in the lymphocy