Possible involvement of chromosome 1 in in vitro immortalization: Evidence from progression of a human adenoma-derived cell line in vitro

✍ Scribed by Christos Paraskeva; Amanda Harvey; Susan Finerty; Susan Powell

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: French
• Weight: 496 KB
• Volume: 43
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910430434

✦ Synopsis

We have previously reported that continuous in vitro passage in the presence of 3T3 feeders of a non-tumorigenic adenoma-derived epithelial cell line, designated PCIAA, resulted in its becoming immortal. At early passage PCIAA was normal diploid, whereas every cell of PCIAA late passage had an isochromosome l(q) which led us to suggest that abnormalities of chromosome I may be involved in tumour progression. We now report the isolation of a 313-feederindependent variant of early-passage PCIAA, designated PCI AA/FI, which was immortal in vitro and remained nontumorigenic. Each cell of PCIAAIFI again has an isochromosome I(q), like the late-passage PCIAA. However, with PCI A N F I it IS the other chromosome I of the homologous pair which is involved in the formation of the isochromosome I(q). This is possible to determine because of the polymorphic centromeric heterochromatin on chromosome I of the earlypassage PCIAA. With the late-passage PCIAA (grown with 3T3 feeders) the homologue with the large C-band has given rise to an isachromosome I(q) whereas with P C I W F I it is the other homologue with the smaller C-band which has given rise to this isochromosome. Both the immortal PCI ANFI and the immortal PCIAA late passage, therefore, have independent abnormalities involving chromosome I. These results indicate that chromosome I may be involved in in vitro immortalization.