Specific cell binding using staphylococcal protein A magnetic microspheres
โ Scribed by Kenneth J. Widder; Andrew E. Senyei; Haim Ovadia; Philip Y. Paterson
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 616 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0022-3549
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๐ SIMILAR VOLUMES
An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated o
We contrived a new separation system using a stirred cell charged with uncoupled microsphere similar to the chromatographic separation. Microspheres, carboxylated PS/PMAA and sulfonated PS/PNaSS, were prepared by emulsifier-free emulsion polymerization. To complement the submicron size weakness and