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Species differences in the effect of parathyroid hormone on cyclic amp production

✍ Scribed by Lewin, I.; Tomlinson, S.; Hendy, G.N.; O'Riordan, J.L.H.; Pickard, D.W.; Care, A.D.

Book ID: 122884527
Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 389 KB
Volume: 67
Category: Article
ISSN: 0300-9629
DOI: 10.1016/S0300-9629(80)80025-9

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## Cell culture The opossum kidney (OK) cells, kindly provided by Dr. J. Handler, National Institutes of Health, Bethesda, Maryland, were seeded at a density of 1.2 x 104/cm2 in DMEM, supplemented with 10% FCS, containing 100 pg/ml of streptomycin and 100 IU/ml of penicillin. Medium was replaced o