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Effect of dexamethasone on parathyroid hormone stimulation of cyclic AMP in an opossum kidney cell line

✍ Scribed by R. Rizzoli; J.-Ph. Bonjour

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
670 KB
Volume
132
Category
Article
ISSN
0021-9541

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✦ Synopsis

Cell culture

The opossum kidney (OK) cells, kindly provided by Dr. J. Handler, National Institutes of Health, Bethesda, Maryland, were seeded at a density of 1.2 x 104/cm2 in DMEM, supplemented with 10% FCS, containing 100 pg/ml of streptomycin and 100 IU/ml of penicillin. Medium was replaced on days 3 and 6 , and 6 hours before the experiments, which were performed on day 9 after plating. Incubations with dexamethasone or its solvent

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