Spacer-mediated display of active lipase on the yeast cell surface
โ Scribed by M. Washida; S. Takahashi; M. Ueda; A. Tanaka
- Publisher
- Springer
- Year
- 2001
- Tongue
- English
- Weight
- 169 KB
- Volume
- 56
- Category
- Article
- ISSN
- 1432-0614
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## Abstract The display of PHB depolymerase (PhaZ~RpiT1~) from __R. pickettii__ T1 on the surface of __E. coli__ JM109 cells is realized using OprI of __P. aeruginosa__ as the anchoring motif. The fusion protein is stably expressed and its surface localization is verified by immunofluorescence micr
## Background For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized enzymes. ## Results Two __Pichia pastoris__ c