✦ LIBER ✦

Sources of error in quantitative paper and thin layer chromatography : I. Production of the initial spots

✍ Scribed by J.W. Fairbairn; S.J. Relph

Publisher: Elsevier Science
Year: 1968
Tongue: English
Weight: 627 KB
Volume: 33
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)98678-3

No coin nor oath required. For personal study only.

✦ Synopsis

A study of the errors involved in measuring small volumes of solution to form the initial spots of a chromatogram has been made. Radioactive solutes were used so that quantities could be measured conveniently and rapidly as radioactivities. Errors of up to 20 o/o were found even with experienced workers using conventional syringes or pipettes. Investigation of this surprisingly large source of error showed it was mainly due to "creep back" up the outside of the needle during repetitive delivery and "capillation" from within the needle when touching the surface of the adsorbent. A machine has been devised to overcome these defects; using it the errors of replicate delivery of small volumes have been reduced to about & 4 %.

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