Sonocatalytic damage of bovine serum albumin (BSA) under ultrasonic irradiation with TiO2/tooth composite

✍ Scribed by Zhiqiu Wang; Ying Li; Jun Wang; Xudong Jin; Mingming Zou; Guangxi Han; Kai Li; Jingqun Gao

• Publisher: Wiley (John Wiley & Sons)
• Year: 2011
• Tongue: English
• Weight: 420 KB
• Volume: 87
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.2741

✦ Synopsis

BACKGROUND:

In order to effectively degrade bovine serum albumin (BSA) under ultrasonic irradiation, biological mineral material (tooth powder) was adopted to mix with nano-sized TiO 2 powder. A TiO 2 /tooth composite with high sonocatalytic activity and remarkable selectivity was prepared. RESULTS: TiO 2 /tooth composite with tooth content of 30% (w/w) heat-treated at 500 • C for 40 min was used as sonocatalyst and the catalytic degradation of BSA under ultrasonic irradiation was examined. Some influencing factors, such as ultrasonic irradiation time, TiO 2 /tooth catalyst amount, solution acidity and NaCl concentration, were studied by UV-vis and fluorescence spectroscopic analysis. Furthermore, the BSA attack site for the TiO 2 /tooth composite was identifies by synchronous fluorescence spectra.

CONCLUSION:

The results indicated that, under ultrasonic irradiation, the TiO 2 /tooth composite can promote the degradation of BSA more effectively than pure nano-sized TiO 2 powder. The attack site is identified as tyrosine (Tyr) residue. These results are of great significance for the use of a sonocatalytic method to treat tumours in clinical applications.