Sonocatalytic damage of bovine serum albumin (BSA) under ultrasonic irradiation by mixed TiO2/SiO2 powder

✍ Scribed by Jun Wang; Na Ding; Zhaohong Zhang; Shixian Wang; Ying Guo; Bin Liu; Yuanyuan Zhang; Xiangdong Zhang

• Publisher: Wiley (John Wiley & Sons)
• Year: 2009
• Tongue: English
• Weight: 341 KB
• Volume: 84
• Category: Article
• ISSN: 0268-2575
• DOI: 10.1002/jctb.2076

✦ Synopsis

Abstract

BACKGROUND: In order to effectively damage some biomolecules under ultrasonic irradiation, a mixed TiO~2~/SiO~2~ powder with high catalytic activity and selectivity was used as a sonocatalyst.

RESULTS: The mixed TiO~2~/SiO~2~ powder heat treated at 450 °C for 30 min was adopted as a sonocatalyst and the damage to BSA molecules under ultrasonic irradiation was assessed. In addition, the effects of such variables as molar ratio of TiO~2~ and SiO~2~, treatment temperature and time, ultrasonic irradiation time, catalyst amount, solution acidity, ionic nature and strength, ultrasonic irradiation power and D~2~O concentration on the damage to BSA molecules were studied by means of UV‐visible and fluorescence spectra. The results showed that the degree of damage was aggravated by an increase in ultrasonic irradiation time, catalyst amount, solution acidity, ultrasonic irradiation power and D~2~O concentration, but was reduced by an increase in ionic strength.

CONCLUSION: The results indicated that the mixed TiO~2~/SiO~2~ powder displayed higher activity and selectivity compared with nano‐sized TiO~2~ and SiO~2~ powders during the sonocatalytic damage of BSA. The extent of the damage decreased in the order TiO~2~/SiO~2~ > nano‐sized TiO~2~ > nano‐sized SiO~2~. These results are of great significance for applying sonocatalytic methods to treat tumours. Copyright © 2008 Society of Chemical Industry