Some properties of chitinase produced by a potentAspergillus carneusstrain

✍ Scribed by A. A. Sherief; M. M. A. El-Sawah; Mohamed A. Abd El-Naby

• Book ID: 104654889
• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 271 KB
• Volume: 35
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00184691

✦ Synopsis

Seven fungal isolates characterized by high chitinolytic activity were isolated from soil a n d identified. Aspergillus carneus in a 7-day-old shaken culture was the most p r o m i s i n g chitinase producer. The use of chitin as a c a r b o n source favoured p r o d u c t i o n of extracellular chitinase enzymes. M a x i m u m chitinase activity was reached at 10 g chitin/1. A n initial p H value of the culture m e d i u m of 5.0 gave the highest chitinolytic activity. Some properties of the crude enzyme p r o d u c e d by A. carneus were studied. M a x i m a l enzyme activity was reached at p H 4.5 a n d 4 0 °C after 30 min. T h e r m a l treatments at 7 0 °C a n d p H 4.5 had the most adverse effect o n enzyme activity.