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Some properties and applications of Superose 6B

✍ Scribed by Torvald Andersson; Lars Hagel

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
274 KB
Volume
141
Category
Article
ISSN
0003-2697

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✦ Synopsis

Applications of a new agarose-based medium for high-performance preparative gel filtration is described. The 30-pm bead size, high pore volume, and simple experimental technique to prepare highly efficient columns make Superose 6B very suitable for preparative purifications of protein mixtures in the molar mass range of IO3 to 4 X 106. Sample load exceeds 100 mg/ cm*, which indicates that gram quantities may be purified on K 50/60 columns. The scale-up procedure from analytical to preparative columns is demonstrated by the separation of serum samples and the industrial purification of an enzyme-antibody conjugate.

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