๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Some physicochemical factors relevant to cellular interactions

โœ Scribed by D. E. Brooks; J. S. Millar; G. V. F. Seaman; P. S. Vassar

Publisher
John Wiley and Sons
Year
1967
Tongue
English
Weight
999 KB
Volume
69
Category
Article
ISSN
0021-9541

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โœฆ Synopsis

Colloidal stability theory is discussed to accomodate the conditions imposed by biological systems. It is shown that to obtain potential curves with secondary minima, Hamaker's constant must be in the range of 1-5 x lo-" ergs. The effect of increasing the dielectric constant is shown in theory to lower the surface potential and electrophoretic mobility but to increase the total energy of interaction. Calculations made from the theory predict the forces between model cells to be ca.

4.0 x lo-' dynes.

By cone-plate shearing of cell aggregates, the most successful of several techniques tried and discussed, at shear rates approaching 1 X lo-' second-' (1.5 x 1 0 -4 dynes) semicomplete disaggregation was achieved although cell disruption was apparent;

analysis of blood viscosity data indicates 5-10 X lo-' dynes are required to separate red cells suspended in plasma. Colloidal stability theory, while not applicable to cell systems associated by special areas of attachment, seems to describe the physicochemical interaction of freely moving or reversibly adherent cells.

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