Some observations on the preparation of aqueous extracts of spores of aspergillus niger
โ Scribed by Bhatnagar, G. M. ;Krishnan, P. S.
- Book ID
- 104763501
- Publisher
- Springer-Verlag
- Year
- 1959
- Weight
- 259 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0003-9276
No coin nor oath required. For personal study only.
โฆ Synopsis
Microorganisms, in general, require special techniques for the preparation of cell-free extracts (HuGo 1954). The rupture of the spore wail appears to be particularly difficult. Whereas some progress has been made with respect to rupture of bacterial spores, information regarding mold spores is scanty. BAJAJ, I)AMLE and K~ISHNA~r (1954) employed sand and pyrex glass powder as abrasives in their studies on the fraetionation of the phosphorylated compounds in the trichloroacetic acid
๐ SIMILAR VOLUMES
The most important means of elucidating the pathway(s) of metabolism is, probably, the actual identification of all the enzymes involved in the postulated pathway. As far as the authors are aware, there has been no systematic attempt to elucidate the metabolic pathways in spores of fungi, although c
The enzyme a c t i v i t y of resting spores of bacteria a n d the biochemical changes occurring during the various phases of their germination have received considerable a t t e n t i o n during recent years (tIALVO~SOi~ 1957; I-IALVO~SOX a n d C~v~ct~ 1957). Continuing their investigations (B~AT-~
I n a previous c o m m u n i c a t i o n we studied the enzyme catalase present in %he resting spores of Aspergillus niger (B~ATXAGA~ and K~ISJ~XA~ 1960). The presen% paper deals with phosphatase(s) which make theh " appearance during germination.
The germination of spores of microorganisms has been studied in detail with reference to bacterial spores (HALvo~so~ and CgvRcI~ 1957). I n f o r m a t i o n regarding the germination of mold spores is, however, incomplete. U n d e r certain conditions, the germination of mold spores is k n o w n to