Some general aspects of fluorimetric determinations
β Scribed by H. Braunsberg; S.B. Osborn
- Publisher
- Elsevier Science
- Year
- 1952
- Tongue
- English
- Weight
- 899 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0003-2670
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β¦ Synopsis
SOME GENERAL ASPECTS OF FLUORIIMETRIC DETERMTNATIONS Fluorimctric methods arc widely used in organic, biological and industrial cbcmistry. They are usually very sensitive but results are often influenced by small changes in experimental conditions and by impurities present. A general understanding of the physical laws involved, and the assumptions on which fluorimetric methods arc based, as well as their limitations, is essential if such methods -IO Refercnccs p. 95.
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A simple fluorimetric assay for the determination of carbamoyl phosphate in tissue extracts is described. In the assay, production of ATP from carbamoyl phosphate and ADP by carbamate kinase is coupled to the formation of NADPH, using glucose, hexokinase, NADP+, and glucose-6-phosphate dehydrogenase