Some aspects of immobilization and reactivation of whole cells

✍ Scribed by F. Wagner; J. Klein

• Book ID: 104759529
• Publisher: Springer Netherlands
• Year: 1979
• Tongue: English
• Weight: 47 KB
• Volume: 45
• Category: Article
• ISSN: 0003-6072
• DOI: 10.1007/bf00403676

✦ Synopsis

Therefore, in this paper only the physical entrapment in porous polymeric matrices of whole microbial cells are dealt with. Three different types of entrapment in polymeric matrices are discussed: 1. Entrapment in ionic polymeric networks by ionic crosslinking of linear polyelectrolytes via multivalent counter ions. 2. Entrapment in covalent polymeric networks by: a. crosslinking copolymerization of water-soluble monomers in a suspension reaction system b. polycondensation of epoxide prepolymers with polyfunctional amines in the presence of a polyelectrolyte. These techniques lead to particles of controlled porosity, size and cell loading with remarkable mechanical strength. In addition, another interesting feature of these biocatalysts is the possibility that the catalyst can be reactivated after deactivation, which occurs during the preparation of the biocatalyst or after a certain time of operation.