Somatic embryogenesis occurred on cotyledons of morphologically abnormal embryos derived from Vigna glabrescens x V. radiata crosses and cultured on Murashige and Skoog (MS) medium without growth regulators. The frequency of 15-17 day old embryos that gave rise to somatic embryos increased from 8% t
β¦ LIBER β¦
Somatic embryogenesis and regeneration ofVigna radiata
β Scribed by P. Sivakumar; R. Gnanam; K. Ramakrishnan; A. Manickam
- Book ID
- 106371901
- Publisher
- Springer Netherlands
- Year
- 2010
- Tongue
- English
- Weight
- 260 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0006-3134
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Conditions for plant regeneration from excised cotyledons of Vigna radiata were studied. Complete plant developed from the uncallused proximal ends of cotyledons on Murashige & Skoog's (MS), Gamborg's (Bs) and 'C' (MS salts + Bsvitamins) basal media. The basal medium 'C' was found to be best for pla