Culture conditions effecting plant regeneration from cotyledons ofVigna radiata(L.) Wilczek

✍ Scribed by Anju Gulati; Pawan K. Jaiwal

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 512 KB
• Volume: 23
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00116082

✦ Synopsis

Conditions for plant regeneration from excised cotyledons of Vigna radiata were studied. Complete plant developed from the uncallused proximal ends of cotyledons on Murashige & Skoog's (MS), Gamborg's (Bs) and 'C' (MS salts + Bsvitamins) basal media. The basal medium 'C' was found to be best for plant regeneration. Regeneration frequency, however, varied with genotype, size, orientation and age of explant and the different plant growth regulators combination in the medium. Addition of cytokinins induced callusing at the proximal ends of cotyledons followed by multiple shoot formation. Out of 6-benzyl aminopurine (BAP), kinetin (KIN), N (A 2 isopentyl) adenine (2iP) and adenine sulphate (AS), only BAP and KIN were found to be more effective in enhancing the frequency of shoot regeneration. BAP at 1 x 10-SM induced maximum (60%) shoot regeneration whereas maximum number of shoots (8 to 9 shoots) per explant was observed with 5 x 10 6 M BAP. Cotyledons excised from two-day old seedlings were most regenerative. The regenerative response of cotyledons decreased when sliced into two equal parts either longitudinally or transversely. Callusing and organogenic differentiation occured only if the petiolar end of cotyledons was in contact with medium. None of the tested treatments were effective in inducing shoot bud differentiation from subcultured callus. Well developed shoots rooted when incubated on half strength MS, MS and MS basal medium supplemented with IAA (5 x 10-6M). The rooted plants were transferred to pots and later established in the field with 60% success.