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Somatic embryogenesis and plant regeneration inMedicago suffruticosa

✍ Scribed by Xiu-Oing Li; Yves Demarly

Publisher
Springer
Year
1996
Tongue
English
Weight
586 KB
Volume
44
Category
Article
ISSN
0167-6857

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✦ Synopsis

A successful procedure has been designed for the regeneration of plantlets from leaf sections of the self-pollinating species, Medicago suffruticosa. Callus growth was promoted by a 4-week culture period on liquid Kao's medium containing 4.9 #M benzyladenine and 4.5 #M 2,4-dichlorophenoxyacetic acid (2,4-D), followed by a 4-day treatment in which the benzyladenine was elevated to 44.4 #M. Shoots/plantlets were observed after 3-4 weeks culture on growth regulator-free agar-solidified medium. Under these conditions, the regeneration frequency from callus was 18% and a histological study showed that this regeneration was through somatic embryogenesis. The growth regulator treatment, with a relatively high concentration of growth regulators (44.4 #M benzyladenine) for a short time period (4 days), is important for inhibiting polyphenol compounds and for stimulating callus growth and plant regeneration.

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