✦ LIBER ✦

Somatic embryogenesis and plant regeneration in garden leek

✍ Scribed by Wang Hong; Pierre Debergh

Publisher: Springer
Year: 1995
Tongue: English
Weight: 945 KB
Volume: 43
Category: Article
ISSN: 0167-6857
DOI: 10.1007/bf00042667

No coin nor oath required. For personal study only.

✦ Synopsis

High frequency plant regeneration via somatic embryogenesis has been induced from in vitro shoot-base cultures of seedlings of garden leek (Allium porrum L.). Four main steps are involved in the procedure using BDS medium:

-shoot multiplication with 17.6 mM benzyladenine; -induction of nodular callus from the in vitro shoot base with 9 mM 2,4-dichlorophenoxyacetic acid; -initiation of embryogenic callus from nodular callus with 9 mM 2,4-dichlorophenoxyacetic acid + 7.6 mM abscisic acid; -plant regeneration from embryogenic callus with 9.8 mM N6-(2-isopentenyl)adenine. The presence of 2,4-dichlorophenoxyacetic acid in the medium and light conditions were shown to be essential for nodular callus induction and somatic embryogenesis. Abscisic acid was not a prerequiste for somatic embryogenesis, but it significantly increased the frequency.

📜 SIMILAR VOLUMES

Plant regeneration via somatic embryogen

Plant regeneration via somatic embryogenesis in ginger

✍ A. Kackar; S. R. Bhat; K. P. S. Chandel; S. K. Malik 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 754 KB

Embryogenic callus cultures of ginger were induced from young leaf segments taken from in vitro shoot cultures. Among the four auxins tested in Murashige & Skoog medium, dicamba at 2.7 IxM was most effective in inducing and maintaining embryogenic cultures. Efficient plant regeneration was achieved

Secondary somatic embryogenesis and plan

Secondary somatic embryogenesis and plant regeneration in cassava

✍ James A. Stamp; Graham G. Henshaw 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 615 KB

Somatic embryos isolated from mature seed-derived cotyledon cultures of cassava (Mannihot esculenta Crantz) underwent direct secondary somatic embryogenesis or plant development under appropriate incubation conditions. Isolated somatic embryos were subjected to a two-stage culture procedure similar

Somatic embryogenesis and plant regenera

Somatic embryogenesis and plant regeneration ofNerium oleander

✍ Isabel Santos; Isabel Guimarães; Roberto Salema 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 1024 KB

Leaf explants of Nerium oleander L. produced masses of callus when both an auxin and a cytokinin were included in the medium. Leaves cultured on the B5 medium of Gamborg et al. supplemented with 2,4-dichlorophenoxyacetic acid (2,4-9; 9.05 ~tM) plus benzyladenine (BA; 4.4 laM) produced callus and pro

Somatic embryogenesis and plant regenera

Somatic embryogenesis and plant regeneration inMedicago suffruticosa

✍ Xiu-Oing Li; Yves Demarly 📂 Article 📅 1996 🏛 Springer 🌐 English ⚖ 586 KB

A successful procedure has been designed for the regeneration of plantlets from leaf sections of the self-pollinating species, Medicago suffruticosa. Callus growth was promoted by a 4-week culture period on liquid Kao's medium containing 4.9 #M benzyladenine and 4.5 #M 2,4-dichlorophenoxyacetic acid

Somatic embryogenesis, plant regeneratio

Somatic embryogenesis, plant regeneration and somaclonal variation in barley

✍ M. L. Ruíz; J. Rueda; M. I. Peláez; F. J. Espino; M. Candela; A. M. Sendino; A. 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 637 KB

Somatic embryogenesis and plant regenera

Somatic embryogenesis and plant regeneration in olive (Olea europaeaL.)

✍ E. Rugini 📂 Article 📅 1988 🏛 Springer 🌐 English ⚖ 688 KB

Leaf discs from olive (Olea europaea L.) grown in vitro and immature zygotic embryos collected at 50, 75, 90 and 105 days after full bloom were tested for their somatic embryogenic capacity. The embryos were grown in half-strength MS medium and halfstrength OM medium with BAP combinated with either