Somatic Embryogenesis and Plant Regeneration from Hypocotyl Cultures of Digitalis obscura L.
β Scribed by Arrillaga, I.; Brisa, M.C.; Segura, J.
- Book ID
- 122560052
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 754 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0176-1617
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A simple protocol has been developed for high frequency protoplast regeneration via somatic embryogenesis in B. napus. Protoplasts isolated from hypocotyl tissue of 8-12 day old seedlings of Brassica napus ISN706 (AACC) when cultured in KM(A) medium resulted in divisions with a, frequency ranging fr
Callus was obtained from mericarps of Bunium persicum Boiss. on MS medium supplemented with 2.0 mg/1 2,4-D and 4.0 mg/1 Kn. Small white clumps of compactly packed cells developed on the callus on a medium containing 1.0 mg/1 2,4-D and 0 mg/1 Kn. These cell clumps differentiated numerous globular emb