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Solvent dependence of pyranine fluorescence and UV-visible absorption spectra

✍ Scribed by Noga Barrash-Shiftan; Brina (Beth) Brauer; Ehud Pines

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 232 KB
Volume: 11
Category: Article
ISSN: 0894-3230
DOI: 10.1002/(sici)1099-1395(1998100)11:10<743::aid-poc39>3.0.co;2-h

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✦ Synopsis

Fluorescence and UV-visible absorption spectra of HPTS (8-hydroxy-1,3,6-pyrenetrisulphonic acid trisodium salt, pyranine) were measured in a variety of solvents. Fluorescence maxima (in kcal mol -1 ) can be correlated with the Kosower Z parameter (r = 0.901), the Dimroth-Reichardt E T (30) parameter (r = 0.900) and the Winstein Y parameter (r = 0.916) using one-parameter fits. Good correlations (r = 0.98) were obtained for HPTS fluorescence in ethanol-water mixtures using the Y, Y OTs and Z parameters. Fluorescence maxima of HPTS in aqueous sulphuric acid solutions gave an excellent correlation with Y OTs (r = 0.991). Multi-parameter correlations, indicating the significance of specific solvent interactions, were also studied. In addition, fluorescence maxima correlate well with maximum/minimum ratios obtained from UV-visible absorption experiments. Results can be applied in the use of HPTS as a molecular probe of solvent environments and for extension of the Y OTs scale in acidic solutions. HPTS is a unique molecular probe, not only because of its photoacidic properties and its widespread use as a pH-sensitive biosensor, but also because of its relative stability in acidic environments.

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